Composite
Part:BBa_J36846:Design
Designed by: Perry Tsai Group: iGEM06_Harvard (2006-10-30)
Lpp-OmpA(46-159)-Streptavidin wild-type + His6
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 485
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 527
Illegal AgeI site found at 578 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.
Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts.
Source
Assembly of J36835, J36837, J36841