Part:BBa_J36846:Design

Lpp-OmpA(46-159)-Streptavidin wild-type + His6

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 485
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 527
  Illegal AgeI site found at 578
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.

Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts.

Source

Assembly of J36835, J36837, J36841